Effect Of Electromagnetic Field On Endocytosis Of Cationic Solid Lipid Nanoparticles By Human Brain-Microvascular Endothelial Cells.

Abstract

This study investigates the electromagnetic field (EMF)-regulated transport of cationic solid lipid nanoparticles (CSLNs) across human brain-microvascular endothelial cells (HBMECs). The positive charge of CSLNs was from dioctadecyldimethyl ammonium bromide and stearylamine, and radiofrequency EMF was applied to HBMECs for promoting uptake of CSLNs. Immunochemical staining revealed that the expression of clathrin on the membrane of HBMECs enhanced during vesicular endocytosis of CSLNs. However, CSLNs and EMF slightly affected the expression of P-glycoprotein on the membrane of HBMECs. An exposure to EMF yielded negligible increase in the permeability of free saquinavir (SQV) across the HBMEC monolayer. Nevertheless, the permeability of SQV across the HBMEC monolayer increased about 17-fold when SQV was entrapped in CSLNs. Moreover, the permeability of SQV across the HBMEC monolayer increased about 22-fold by applying the CSLN encapsulation and EMF exposure. CSLNs and EMF could produce synergistic effect on improving the brain-targeting delivery.

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Microwave Frequency Electromagnetic Fields (Emfs) Produce Widespread Neuropsychiatric Effects Including Depression

Abstract

Non-thermal microwave/lower frequency electromagnetic fields (EMFs) act via voltage-gated calcium channel (VGCC) activation. Calcium channel blockers block EMF effects and several types of additional evidence confirm this mechanism. Low intensity microwave EMFs have been proposed to produce neuropsychiatric effects, sometimes called microwave syndrome, and the focus of this review is whether these are indeed well documented and consistent with the known mechanism(s) of action of such EMFs. VGCCs occur in very high densities throughout the nervous system and have near universal roles in release of neurotransmitters and neuroendocrine hormones. Soviet and Western literature shows that much of the impact of non-thermal microwave exposures in experimental animals occurs in the brain and peripheral nervous system, such that nervous system histology and function show diverse and substantial changes. These may be generated through roles of VGCC activation, producing excessive neurotransmitter/neuroendocrine release as well as oxidative/nitrosative stress and other responses...

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Court Case: Official Recognition Of James Walbert’s Case+support From Missouri Rep.jim Guest

Wichita, KS April 20, 2009

A Wichita inventor who claims he is being attacked by directed high energy microwave weapons, victimized by covert organized stalking and targeted by highly advanced forms of electronic harassment was granted an order of protection against one of his assailants, according to a Sedgwick County District Court ruling. After reviewing a petition filed by victim James Walbert, Judge Terry Pullman determined the case should be weighed on its merits and a hearing was scheduled before Judge James Beasley. In the end, Walbert prevailed.

Walbert’s evidence included declassified government documents, photos and scientific data attesting to the authenticity of land-based and satellite-based directed microwave weaponry. His proof also included a radiation forensics report concluding Walbert is being targeted with obscure microwave frequencies. And, to show he is not the only victim of these crimes, Walbert provided a letter from Missouri State Representative James O. Guest corroborating that his office has...

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